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A novel technique for retrospective genetic analysis of the response to vaccination or infection using cell-free DNA from archived sheep serum and plasma

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Date
05/02/2020
Author
Hanks, E.
Todd, H.
Palarea Albaladejo, J.
McNeilly, T.
Britton, C.
Ballingall, K.T.
Publisher
BMC
Version
2020-02-05
2020-02-05
Metadata
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Abstract
Genetic variation is associated with differences in disease resistance and susceptibility among individuals within a population. To date, molecular genetic analyses of host responses have relied on extraction of genomic DNA from whole blood or tissue samples. However, such samples are not routinely collected during large‐scale field studies. We demonstrate that cell‐free genomic DNA (cfDNA) may be extracted and amplified from archived plasma samples, allowing retrospective analysis of host genetic diversity. This technique was also applicable to archived serum samples up to 35 years old and to different ruminant species. As proof of concept, we used this cfDNA approach to genotype the major histocompatibility complex (MHC) class II DRB1 locus of 224 Merino sheep which had participated in field trials of a commercial Haemonchus contortus vaccine, Barbervax DRB1 alleles and their relative frequencies. This is the first study to examine host MHC diversity using DNA extracted from archived plasma samples, an approach that may be applied to retrospective analyses of genetic diversity and responses to vaccination or infection across different species and populations.
DOI
https://doi.org/10.1186/s13567-020-0737-9
Link
https://hdl.handle.net/20.500.12594/10014
Citation
Veterinary Research, 51, 9
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©Research Scotland Consortium
c/o RGBE 20a Inverleith Row
EH3 5LR
Edinburgh, Scotland, UK

Tel: 0131 248 2850
Email: info@ResearchScotland.ac.uk
Items in Research Scotland are protected by copyright with all rights reserved unless otherwise indicated.
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